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Open Access Research

3p21.3 tumor suppressor gene RBM5 inhibits growth of human prostate cancer PC-3 cells through apoptosis

Lijing Zhao24, Ranwei Li3, Chen Shao24, Ping Li1, Jian Liu2 and Ke Wang1*

Author Affiliations

1 Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, Jilin, 130041, China

2 Department of Pathophysiology, Norman Bethune College of Medicine of Jilin University, Changchun, Jilin, 130021, China

3 Department of Urinary Surgery, The Second Affiliated Hospital of Jilin University, Changchun, Jilin, 130041, China

4 Department of Digestive Medicine, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, China

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World Journal of Surgical Oncology 2012, 10:247  doi:10.1186/1477-7819-10-247

Published: 16 November 2012

Abstract

Background

Recent studies have indicated that the nuclear RNA-binding protein RBM5 has the ability to modulate apoptosis and suppress tumor growth. The aim of this study is to investigate the expression of RBM5 in human prostate cancer and its mechanism of tumor suppression.

Methods

The expression of RBM5 protein in cancerous prostatic tissues and normal tissues was examined by IHC. PC-3 cell line was used to determine the apoptotic function of RBM5 in vitro. PC-3 cells were transiently transfected with pcDNA3.1-RBM5. Cell viability was determined by MTT assay. Rhodamine 123 staining and Annexin V analysis were performed to observe the apoptotic activity of PC-3 cells overexpressing RBM5. Expression of apoptosis-related genes was assessed by western blot.

Results

The expression of RBM5 protein was significantly decreased in cancerous prostatic tissues compared to the normal tissues. PC-3 cells overexpressing RBM5 showed not only significant growth inhibition compared with the vector controls, but also dysfunction of mitochondrial membrane potential and increased apoptotic activity. To further define RBM5 function in apoptotic pathways, we investigated differential expression profiles of various BH3-only proteins including Bid, Bad, and Bim, and apoptosis regulatory proteins include P53, cleaved caspase9, and cleaved caspase3. We found that the expression of both BH3-only proteins and apoptosis regulatory proteins was increased in RBM5 transfected cells.

Conclusion

The expression of RBM5 protein was significantly decreased in cancerous prostatic tissues, which suggests that RBM5 plays an important role in the pathogenesis of prostate cancer. RBM5 may induce the apoptosis of prostate cancer PC-3 cells by modulating the mitochondrial apoptotic pathway, and thus RBM5 might be a promising target for gene therapy on prostate cancer.

Keywords:
RBM5; Prostate cancer; Apoptosis; PC-3; Gene expression