|
Sources, dilution and pretreatment of antibodies used |
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| Antibody |
Clone |
Manufacturer |
Dilution |
Pretreatment |
|
|
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| ER |
1D5 |
DakoCytomation, USA |
1:50 |
boiling (pH9.0, 40 min) |
| PgR |
PgR636 |
DakoCytomation, USA |
1:800 |
boiling (pH9.0, 40 min) |
| HER2 |
DakoCytomation, USA |
Prediluted (Hercep test) |
boiling (pH6.0, 40 min) |
|
| CK5/6 |
D5/16B4 |
DakoCytomation, Denmark |
1:100 |
autoclave (pH6.0, 10 min) |
| CK14 |
LL002 |
NeoMarkers, USA |
1:100 |
autoclave (pH6.0, 10 min) |
| CK17 |
E3 |
DakoCytomation, Denmark |
1:40 |
autoclave (pH6.0, 10 min) |
| EGFR |
2-18C9 |
DakoCytomation, USA |
Prediluted (pharmDX kit) |
proteinase K (room temperature, 5 min) |
| AE1/AE3 |
AE/AE3 |
DakoCytomation, Denmark |
1:50 |
pronase (37 C, 15 min) |
| CK7 |
OV-TL12/30 |
DakoCytomation, Denmark |
1:50 |
pronase (37 C, 15 min) |
| CK8 |
35βH11 |
DakoCytomation, USA |
1:50 |
pronase (37 C, 15 min) |
| CK19 |
RCK108 |
DakoCytomation, Denmark |
1:50 |
autoclave (pH6.0, 10 min) |
| α-SMA |
1A4 |
DakoCytomation, Denmark |
1:100 |
|
| P63 |
4A4 |
DakoCytomation, Denmark |
1:50 |
autoclave (pH6.0, 10 min) |
| GFAP |
6F2 |
DakoCytomation, Denmark |
1:100 |
|
Hirose et al. World Journal of Surgical Oncology 2008 6:60 doi:10.1186/1477-7819-6-60 |
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