GSK-3β and FKHR phosphorylation following CCL25 treatment. MDA-MB-231 cells were tested for their ability to phosphorylate GSK-3β and FKHR following treatment with CCL25, cisplatin and specific-kinase inhibitors (wortmannin and PF-573, 228). In situ total and active (phosphorylated) GSK-3β and FKHR levels were quantified by Fast Activated Cell-based ELISA (FACE) assay before (0 minutes) or after (5 or 10 minutes) CCL25 stimulation in the presence of cisplatin and kinase inhibitors.
Johnson-Holiday et al. World Journal of Surgical Oncology 2011 9:46 doi:10.1186/1477-7819-9-46