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1: Cancer Res. 1994 Jul 15;54(14):3700-2.
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Secretion of bioactive granulocyte-macrophage colony-stimulating factor by human colorectal carcinoma cells.

Lahm H, Wyniger J, Hertig S, Yilmaz A, Fischer JR, Givel JC, Odartchenko N.

Department of Cellular Biology, Swiss Institute for Experimental Cancer Research, Epalinges.

Secretion of several cytokines by colorectal carcinoma cells has been substantiated. These do not include granulocyte-macrophage colony-stimulating factor (GM-CSF) thus far. We show that the supernatant of two human colorectal carcinoma cell lines, LS1034 and SW480, stimulates proliferation of GM-CSF-dependent M07e cells. The activity was constitutively secreted by LS1034 cells and could be induced by serum-free culture conditions in SW480 cells. Addition of a neutralizing anti-GM-CSF antibody completely inhibited this activity. Preabsorption with anti-GM-CSF antibody removed all M07e growth-stimulating activity from LS1034 and SW480 supernatant. Western blot analysis revealed the presence of GM-CSF in LS1034 supernatant. Our results indicate that human colorectal carcinoma cells secrete indeed biologically active GM-CSF.

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PMID: 8033086 [PubMed - indexed for MEDLINE]